Part:BBa_K2036023:Design

RBS-iLDH-TT-pRM-RBS-beta-galactosidase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 737
  Illegal BamHI site found at 776
  Illegal BamHI site found at 1468
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

patp2 are active within pH7~9, so CI will be produced to inhibit pR thus we can regulate CIII's expression by PH level.

Source

patp2 are from Corynebacterium glutamicum ATCC 13032, CI, CIII and pR are all from bacteriaphage lambda.